Chromium chip b single cell kit. Single Cell 3’ Reagent Kits v2 User Guide .
Chromium chip b single cell kit Minimum 10,000 read pairs/cell for gene expression; 5,000 read Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234 Chromium Next GEM Chip J Single Cell Kit, 16 rxns PN-1000230 Single Index Kit N Set A, 96 rxns PN-1000212 Dual Index Kit TT Set A, 96 rxns PN-1000215 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. GemCode Technology samples a pool of ~3,500,000 10x Barcodes to separately index e. Chromium GEM-X Single Cell 5' Chip Kit v3, 4 chips (PN1000698) Reagents . 1 Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1. Chromium Next GEM Automated Chip G Single Cell Kit, 48 rxns PN-1000136; Chromium Next GEM Automated Chip G Single Cell Kit, 16 rxns PN-1000146; Single Index Kit T Set A, 96 rxns PN-1000213; Dual Index Kit TT Set A, 96 rxns PN-1000215; Document Type User Guide. Benchtop centrifuge with 8 Target Enrichment Kits SPECIES PART NUMBER ENRICHMENT KITS & TARGET (PN) Chromium Single Cell V(D)J Human Enrichment Kit, Human T Cell, 1000005 T Cells 96 rxns Chromium Single Cell V(D)J Human Enrichment Kit, Human B Cell, 1000016 B Cells 96 rxns Chromium Single Cell V(D)J Mouse Enrichment Kit, Mouse T Cell, 1000071 T Cells 96 rxns 10xGenomics. Single cells were suspended in PBS containing 0. FOR USE WITH Chromium ™ Single Cell 3’ Library, Gel Bead & Multiplex Kit, 16 rxns PN-120233 Chromium™ Single Cell 3’ Chip Kit PN The 10X Genomics Chromium Single Cell 3’ GEM Library and Gel Bead Kit v3 enables simultaneous library preparation of hundreds to thousands of individual cells for 3’ digital gene Superior reproducibility and time-saving automated single cell partitioning. Single Index Kit N Set A, 96 rxns PN-1000212. This is Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234. 1, 4 rxns PN-1000325 Chromium Next GEM Chip L Single Cell Kit, 16 rxns PN-1000321. 1 is a high throughput solution on the Chromium X series instrument for analyzing hundreds of thousands of cells per run, with up to 16 samples in a single chip. Chromium Chip C Single Cell DNA Kit, 48 rxns PN-1000022 (store at ambient temperature) Chromium Chip D Single Cell DNA Kit, 24 rxns PN-1000042 (includes PN-1000021, 2 kits) (store at ambient temperature) Chromium i7 Multiplex Kit, 96 rxns PN-120262 (store at Partitioning oil (from Chromium Chip B Single Cell Kit). 1 FOR USE WITH Chromium Next GEM Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3. 1 (Dual Index) FOR USE WITH. 1 Gel Beads In addition to a poly(dT) primer sequence that enables the production of barcoded, full-length cDNA from polyadenylated mRNA, Single Cell 3’ v3. 1, 4 rxns PN-1000325 3’ Feature Barcode Kit, 16 rxns PN-1000262 Chromium Next GEM Chip L Single Cell Kit, 16 rxns PN-1000321 Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3. Chromium Next GEM Chip H Single Cell Kit, 48 rxns PN-1000161 Chromium Next GEM Chip H Single Cell Kit, 16 rxns PN-1000162 Single Index Kit N, Set A, 96 rxns PN-1000212 Next GEM CG000399 Rev B. 0. 1, 16 rxns PN-1000121 Chromium Next GEM Single Cell 3 ʹ GEM Kit v3. 19. com CG000204 Rev D USER GUIDE Chromium Next GEM Single Cell 3ʹ Reagent Kits v3. Chromium™Single Cell Controller, 24 Mo. 1, 16 rxns PN-1000268 Chromium Next GEM Single Cell 3 ’ Kit v3. Click to TOC Chromium Single Cell A Chip Kit, 48 rxns PN-120236 Chromium Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium i7 Multiplex Kit, 96 rxns PN-120262 . Next GEM. libraries generated using the Chromium Single Cell ATAC Reagent Kit protocol. This document outlines the protocol to generate an enriched T-cell library and/or an enriched B-cell library, and/or a 5’ G. 1, 48 rxns PN-1000161 (store at ambient temperature) Chromium Next GEM Chip H Single Cell Kit v1. The kit is designed for single-cell isolation, sample preparation, and downstream analysis. com Chromium Next GEM Chip K & Gaskets Chromium Next GEM Chip K 6 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120; Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127; Dual Index Kit TT Set A, 96 rxns PN Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. 1 workflow. com CG000183 Rev A USER GUIDE Chromium Single Cell 3ʹ Reagent Kits v3 FOR USE WITH Chromium Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3, 16 rxns PN-1000075 Chromium Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3, 4 rxns PN-1000092 Chromium Single Cell B Chip Kit, 48 rxns PN-1000073 Chromium Next GEM Chip H Single Cell Kit, 16 rxns PN-100016 2 (store at ambient temperature) Single Index Kit N Set A, 96 rxns PN-1000212 (store at −20°C) Introduction. 1 (Dual Index) with Feature Barcode technology for Cell Surface Protein FOR USE WITH Chromium Next GEM Single Cell 3 ʹ LT Kit v3. Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Click to TOC Chromium Next GEM Single Cell V(D)J Reagent Kits v1. This library was initially sequenced using paired-end sequencing (26bp Read 1 and 98bp Read 2) with a single sample index (8bp) on an Illumina HiSeq 4000 with approximately 93,000 reads per cell (two lanes). 1, 16 rxns PN-1000175 ATAC Buffer B 1 2000193 ATAC Enzyme 1 2000123 20X Nuclei Buffer 1 2000207 Barcoding Reagent B 1 2000194 Barcoding Enzyme 1 2000125 SI-PCR Primer B 1 2000128 Reducing Agent B 1 2000087 Amp Mix 1 2000047 Chromium Single Cell A Chip Kit, 48 rxns PN-120236 Chromium Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium i7 Multiplex Kit, 96 rxns PN-120262 Chromium i7 Multiplex Kit N, Set A, 96 rxn PN-1000084. report a method for paired, single-cell whole-genome sequencing and ATAC-seq using kits on the widely used 10× Genomics Chromium platform. 1 16 rxns 4 rxns 1000121 1000128 Chromium Next GEM Chip G Single Cell Kit 48 rxns 16 rxns 1000120 1000127 Chromium Next GEM Single Cell 3’ Library Chromium Chip E Single Cell ATAC Kit, 48 rxns PN-1000082 Chromium Chip E Single Cell ATAC Kit, 16 rxns PN-1000086 Chromium i7 Multiplex Kit N, Set A, 96 rxns PN-1000084. Chromium Next GEM Training Chip 6 2000201 Gaskets, 6-pack 1 370017 Partitioning Oil 6 2000190 Chromium Chips & Gaskets # PN Recovery Agent 6 220016 10xGenomics. Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NN Set A, 96 rxns PN-1000243 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. com Partitioning Oil 2 220088 Chromium Chip C & Gaskets # PN To produce GEMs , the 10× barcoded gel beads are combined with the 10× genomics master mix, sorted cells of interest (CD8a + T cells, as detailed in this chapter), and partitioning oil; then added onto the 10× Genomics Chromium Chip B, as designated in the Chromium Single Cell 3′ Solution Reagents Kits v3 user guide; and placed into the Chromium Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NN Set A, 96 rxns PN-1000243 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. 1, 4 rxns PN-1000147 Chromium Next GEM Automated Chip G Single Cell Kit, 48 rxns PN-1000136 Chromium Chip C Single Cell DNA Kit, 16 rxns PN-1000032 (store at ambient temperature) Click to TOC Chromium Single Cell DNA Training Kit User Guide | Rev A 7 Chromium Single Cell DNA Accessory Kit, PN-1000058 Introduction Flowmi 1 1000055 # PN Recommended Thermal Cyclers Supplier Description Part Number Chromium Automated Single Cell Human TCR Amplification & Library Construction Kit, Chromium Next GEM Chip K Automated Single Cell Kit, 48 rxns PN-1000289 Chromium Next GEM Chip K Automated Single Cell Kit, 16 rxns PN-1000297 Dual Index Kit Loading the Single Cell 3’ Chip 15 2. 1 (-80°C Freezer) Chromium Next GEM Chip G Single Cell Kit (Room Temp) Single Index Kit T Set A OR Dual Index Kit TT Set A (-20°C Freezer) Not included in the 10X kits: Cell Suspension (See following Chromium™ Single Cell 3’ Chip Kit v2 PN-120236 Chromium™ i7 Multiplex Kit PN-120262. About us; Investors; Careers; Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Single Index Kit T Set A, 96 rxns PN-1000213 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. FOR USE WITH Chromium Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237 Chromium Single Cell 3’ Library & Gel Bead Kit v2, 4 rxns PN-120267 Chromium Single Cell A Chip Kit, 48 rxns PN-120236 Chromium Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium Next GEM Chip Q Single Cell Kit, 48 rxns PN-1000418 / 16 rxns PN-1000422; Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 1 BC PN-1000474; Chromium Fixed RNA Kit, Mouse Transcriptome, 4 rxns x 1 BC PN-1000495; Fixed RNA Feature Barcode Kit 16 rxns PN-1000419; Chromium ™ Single Cell Controller & Accessory Kit PN-120263 . 0 or higher on Chromium X/iX is required for running this assay. 1 Gel Beads include two additional primer sequences (Capture Sequence 1 and Capture Sequence 2) for direct capture and priming of Feature Barcode Chromium Chip D Single Cell DNA Kit, 48 rxns PN-1000042 (store at ambient temperature) Chromium i7 Multiplex Kit, 96 rxns PN-120262 (store at −20°C) Introduction Chromium Chip D Single Cell DNA Kit, PN-1000021 (2 kits are shipped) Click to TOC Chromium Single Cell DNA Reagent Kits User Guide | Rev B 12 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3. Click to TOC Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. 1, 4 rxns PN-1000269 3’ Feature Barcode Kit, 16 rxns PN Chromium Single Cell A Chip Kit, 48 rxns PN-120236 Chromium Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium i7 Multiplex Kit, 96 rxns PN-120262. Chromium™ Single Cell V(D)J Enrichment Kit, Human B Cell (store at −20°C) 1000016 Human B Cell Mix 1 6 2000035 Human B Cell Mix 2 6 2000036 Chromium™ Single Cell A Chip Kits PN Chromium™ Single Cell 3’ Reagent Kits User Guide . 01 per cell; Streamlined multiplexing for flexible scaling & higher efficiency. 1, 4 rxns PN Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234. 2. Click to TOC Chromium Single Cell ATAC Reagent Kits User Guide | Rev B 2 Notices Document Number CG000168 | Rev B Chromium Single Cell V(D)J Enrichment Kit, Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120; Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127; Single Index Kit T Set A, 96 rxns PN-1000213; Important. Chromium Single Cell Controller Readiness Test – CG00051. 1 (Document CG000209) and Chromium Next GEM Single Cell ATAC Reagent Kits v2 (Document CG000496) User Guides. Single Cell ATAC Reagent Kits. Sequencing requirements. 1 Gel Beads include two additional primer sequences (Capture Sequence 1 and Capture Sequence 2) for direct capture and priming of Feature Barcode 3’ CellPlex Kit). com Chromium Next GEM Chip K & Gaskets Chromium Next GEM Chip K 6 2000182 Gasket, 6-pack 1 370017 # PN. Company. The location of the rows labeled 1-3 and J Enrichment Kit, Mouse B Cell Chromium Single Cell V(D)J Enrichment Kit, Mouse T Cell 96 rxns 96 rxns 96 rxns 96 rxns 1000016 1000005 1000072 1000071 Chromium Next GEM Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Chromium Next GEM Chip J Single Cell Kit, 16 rxns PN-1000230 Single Index Kit N Set A, 96 rxns PN-1000212. Chromium i7 Multiplex Kit, 96 rxns PN-120262. Click to TOC Chromium Single Cell V(D)J Reagent Kits User Guide with Feature Barcoding technology for Cell Surface Protein • Rev B 2 CG000390 Rev B USER GUIDE Chromium Next GEM Single Cell 3’ Reagent Kits v3. Chromium Next GEM Chip H Single Cell Kit: 48 rxns 16 rxns: 1000161 1000162: Single Index Kit N, Set A 96 rxns: 1000212 INSTRUMENT: Chromium Controller & Next GEM Accessory Kit - 120223 (12 month warranty) The 10X Genomics Chromium Single Cell 3’ GEM Library and Gel Bead Kit v3 enables simultaneous library preparation of hundreds to thousands of individual cells for 3’ digital gene expression profiling analysis. 1, 24 rxns PN-1000141 Chromium Next GEM Automated Single Cell 3’ Library and Gel Bead Kit v3. 1: Reagents, Workflow & Data Overview Introduction Chromium Next GEM Single Cell 3' HT v3. Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127; Single Index Kit T Set A, 96 rxns PN-1000213; Chromium Single Cell 5' Feature Barcode Library Kit, 16 rxns PN-1000080; Single Index Kit N Set A, 96 rxns PN-1000212; Important. Chromium Single Cell V(D)J Enrichment Kit, Human B Cell, 96 rxns PN-1000016 Chromium Single Cell V(D)J Enrichment Kit, Mouse T Cell, 96 rxns PN-1000071 Chromium Single Cell V(D)J Enrichment Kit, Mouse B Cell, 96 rxns PN-1000072 Chromium Single Cell A Chip Kit, 48 rxns PN-120236 Chromium Single Cell A Chip Kit, 16 rxns PN-1000009 Queitsch et al. Dual Index Kit TT Set A 96 rxns. 1 FOR USE WITH Chromium Next GEM Single Cell 3 ʹ GEM, Library & Gel Bead Kit Chromium. 2. 1 # RT Reagent B 1 2000165 RT Enzyme C 1 2000102 Template Switch Oligo 1 3000228 Reducing Agent B 1 2000087 Cleanup Buffer 1 2000088 cDNA Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 Dual Index Kit TT Set A, 96 rxns PN-1000215 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Chromium Next GEM Chip K Automated Single Cell Kit, 16 rxns PN-1000297 Dual Index Kit TT Set A, 96 rxns PN-1000215. FOR USE WITH Chromium ™ Single Cell 3’ Library, Gel Bead & Multiplex Kit, Chromium™ Single Cell 3’ Chip Kit (store at ambient temperature) 120232 . Chromium Next GEM Single Cell 3' HT v3. Single Cell 3’ Reagent Kits v2 User Guide . 1, 4 rxns PN-1000269 Chromium Next GEM Single Cell 3 ’ GEM Kit v3. com ChromiumTM Single Cell 3’ v2 Cell Suspension Volume Table Cell Suspension Volume Table – Grey boxes: Volumes that would exceed the allowable water volume in each reaction Chromium Single Cell B Chip Kit, 16 rxns PN-1000074 (Europe, Middle East and Africa) Chromium i7 Multiplex Kit, 96 rxns PN-120262 Upgrading to Firmware 3. com Chromium Next GEM Chip K & Gaskets Chromium Next GEM Chip K 6 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. It enables the capture and barcoding of individual cells, allowing for the study of gene g cells, and Partitioning Oil onto Chromium Chip B. CG000399 Rev B. ch cell’s transcriptome and cell surface Upgrading to Firmware 4. Chromium Next GEM Single Cell 3 ’ Kit v3. GemCode Technology samples a pool of ~3,500,000 10x Barcodes to separately index each cell’s transcrip. Last Modified July 29, 2024. Chromium . 1 (Dual Index) with Feature Barcode technology for CRISPR Screening FOR USE WITH Chromium Next GEM Single Cell 3 ’ Kit v3. Dual Index Kit TS Set A, 96 rxn. FOR USE WITH Chromium ™ Single Cell 5’ Library & Gel Bead Kit, 16 rxns PN-1000006 Chromium™ Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005 Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236 Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium Next GEM Automated Single Cell 3’ Reagent Kits v3. Index Kit. Rainin pipette tips have been extensively validated by 10x G. Chromium Next GEM Chip K Automated Single Cell Kit, 16 rxns PN-1000297 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit TN Set A, refer to Chromium Next GEM Single Cell 5' cDNA Kit v2 User Guide Supplement (CG000473) and Automated Library Construction User Guide (CG000474). The Chromium Single Cell platform was designed to help make single cell studies accessible and approachable for everyone. 1 16 rxns 4 rxns 1000121 1000128 Chromium Next GEM Chip G Single Cell Kit 48 rxns 16 rxns 1000120 1000127 Chromium Next GEM Single Cell 3’ Library CG000400 Rev B USER GUIDE Chromium Next GEM Single Cell 3ʹ LT Reagent Kits v3. Click to TOC Chromium Single Cell V(D)J Reagent Kits User Guide | Rev J 2 Notices Document Number CG000086 | Rev J Chromium Next GEM Single Cell ATAC Reagent Kits v1. 48 rxns PN-1000418 | 16 rxns PN-1000422. 1, 4 rxns PN-1000269 3’ Feature Barcode Kit, 16 rxns PN-1000262 3’ CellPlex Kit Set A, 48 rxns PN-1000261 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234. 1(Dual Index) User Guide • Rev D 2 Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3. Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234 Chromium Next GEM Chip J Single Cell Kit, 16 rxns PN-1000230 Single Index Kit N Set A, 96 rxns PN-1000212 Dual Index Kit TT Set A, 96 rxns PN-1000215 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Click to TOC Demonstrated Protocol – Chromium Single Cell V(D)J Reagent Kits – Direct Target Enrichment | Rev A 2 Notices Document Number 10xGenomics. Click to TOC Chromium Single Cell 3 Reagent Kits v3 User Guide with Feature Barcoding technology for Cell Surface Protein | Chromium Next GEM Chip K Automated Single Cell Kit, 16 rxns PN-1000297 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit TN Set A, Chromium Next GEM Automated Single Cell 5' Reagent Kits v2 17 Chromium Next GEM Automated 5' Quick Planner Card 27 Tips & Best Practices 28 Consumables 29 Cell Concentration 29 Chromium Next GEM Chip H Single Cell Kit, 48 rxns PN-1000161; Chromium Next GEM Chip H Single Cell Kit, 16 rxns PN-1000162; Single Index Kit N, Set A, 96 rxns PN-1000212; Important. 1 or v2 biochemistry. Single Cell 3’ Chips . About us; Investors; Careers; Contact; News; Distributors; Platforms. Chromium Next GEM Single Cell ATAC Reagent Kits v1. Cell suspensions (containing 800 to 16,000 cells per sample) are loaded into a Chromium Chip B along with partitioning oil, Chromium Chip E Single Cell ATAC Kit, 16 rxns PN-1000156 (America & Asia Pacific), PN-1000086 (Europe, Middle East & Africa) Chromium i7 Multiplex Kit N, Set A, 96 rxns PN-1000084 Available for use only with the indicated Chromium Controller (PN-120223;120246) or Chromium Single Cell Controller (PN-120263; 120212). 1 (-20°C Freezer) Library Prep Kit (-20°C Freezer) Chromium Next GEM Single Cell Gel Bead Kit v3. Click to TOC Chromium Next GEM Single Cell 5' v2 (Dual Index) User Guide Rev D 12 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. Chromium Next GEM Chip H Single Cell Kit: 48 rxns 16 rxns: 1000161 1000162: Single Index Kit N, Set A 96 rxns: 1000212 INSTRUMENT: Chromium Controller & Next GEM Accessory Kit - 120223 (12 month warranty) CG000400 Rev B USER GUIDE Chromium Next GEM Single Cell 3ʹ LT Reagent Kits v3. 1, 16 rxns PN-100016 2 (store at ambient temperature) Single Index Kit N Set A, 96 rxns PN-1000212 (store at −20°C) Introduction. REAGENT. 1 (for addititional libraries) 16 rxns: Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. Dual Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236 Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium™ i7 Multiplex Kit, 96 rxns PN-120262 . Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127. • Before running Single Cell Chips in the Chromium Single Cell Controller, the Readiness Test should be run according to instructions provided in . Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit TN Set A, 96 rxns PN-1000250 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Chromium Single Cell B Chip Kit, 16 rxns PN-1000154 (America & Asia Pacific), PN-1000074 (Europe, Middle East & Africa) Chromium i7 Multiplex Kit, 96 rxns PN-120262. 1, 4 rxns PN-1000147 Chromium Next GEM Automated Chip G Single Cell Kit, 48 rxns PN-1000136 Chromium GEM-X Single . Chromium Next GEM Single Cell 3ʹ GEM v3. Chromium Single Cell 3' v3. ʹ. Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Single-cell RNA-seq libraries were constructed using a Single Cell 3′ Library and Gel Bead Kit (v3. These fast, efficient workflows reduce technical errors and batch effects Chromium™ Single Cell 3’ Reagent Kits v2 User Guide • Rev B Click to TOC ix Additional Kits, Reagents & Equipment The items in the table below have been validated by 10x Genomics® We use the 10X Chromium Controller platform to encapsulate and barcode single cells. Chromium Next GEM Single Cell 5' cDNA Kit v2 User Guide Supplement (CG000473) and Automated Library Construction User Guide (CG000474). LT Kit v3. Fixed RNA Feature Barcode Kit. 1 (Dual Index) User Guide Rev C 10 Refer to the Chromium Single Cell 3ʹ Reagent Kits v3 User Guide for the complete protocol. Dual Index Kit TT Set A. 1; 10X Genomics, 1000121) and a Chromium Single Cell A Chip Kit (10X Genomics, 1000120) according to the manufacturer’s instructions. USER GUIDE Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286 (store at ambient temperature) Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. A. It provides Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 (store at ambient temperature) Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120; Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127; Dual Index Kit TT Set A, 96 rxns PN 10xGenomics. FOR USE WITH Chromium Single Cell ATAC Library & Gel Bead Kit, 16 rxns PN-1000 110 Chromium Single Cell ATAC Library & Gel Bead Kit, 4 rxns PN-1000 111 Chromium Chip E Single Cell ATAC Kit, 48 rxns PN-1000082 Chromium Chip E Single Cell ATAC Kit, 16 rxns PN-1000086 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. 5. . 1 (Dual Index) with Feature Barcode technology for CRISPR Screening and Cell Multiplexing FOR USE WITH Chromium Next GEM Single Cell 3 ’ Kit v3. 3. To achieve single cell resolution, cells are delivered at a limiting dilution, such that the majority (~90-99%) of generated GEMs contain no The Chromium Chip B Single Cell Kit is a laboratory equipment product from 10x Genomics. NOTICES _____ Chromium™ Single Cell 3’ Reagent Kits v2 User Guide Click to TOC• Rev D i Notices from a healthy donor by following the Chromium Single Cell 3’ Reagent Kits v2 User Guide – CG00052. Gaskets . USER GUIDE GEM generation in Chromium Next GEM Chip G (B). Safety data sheet . Chromium Next GEM Single Cell 3 Reagent Kits v3. NOTICES _____ Chromium™ Click to TOCSingle Cell 3’ Reagent Kits v2 User Guide • Rev B i Notices Chromium™ Single Cell V(D)J Enrichment Kit, Human B Cell (store at −20°C) 1000016 Human B Cell Mix 1 6 2000035 Human B Cell Mix 2 6 2000036 Chromium™ Single Cell A Chip Kits PN-120236 & PN-1000009 Product Components # Part Number Chromium™ Single Cell A Chip Kit, 48 rxns (store at ambient temperature) 120236 Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286; Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287; Dual Index Kit TT Set A, 96 rxns PN-1000215; Document Type User Guide. Here, a high-throughput platform is presented, capable of efficient single-cell isolation and multi-color exosome phenotype analysis, as well as quantifying trace exosomes Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234. com CG000204 Rev B USER GUIDE Chromium Next GEM Single Cell 3ʹ Reagent Kits v3. Click to TOC Chromium Single Cell 3 Reagent Kits v3 User Guide with Feature Barcode technology for Cell Surface Protein Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. REACH: Regulation (EC) No 1907/2006 (in particular as Chromium Single Cell V(D)J Enrichment Kit, Mouse B Cell, 96 rxns PN-1000072 Chromium Single Cell A Chip Kit, 48 rxns PN-1000152 (America & Asia Pacific), PN-120236 (Europe, Middle East & Africa) Chromium Single Cell A Chip Kit, 16 rxns PN-1000151 (America & Asia Pacific), PN-1000009 (Europe, Middle East & Africa) Chromium Next GEM Chip Q Single Cell Kit. 1, 16 rxns PN-100016 2 (store at Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient temperature) Chromium i7 Multiplex Kit, 96 rxns PN-120262 (store at −20°C) Introduction: Chromium Chromium. RT Reagent B 1 2000165 RT Enzyme C 1 2000102 Template Switch Oligo 1 3000228 Chromium Next GEM Single Cell ATAC Reagent Kits v1. FOR USE WITH Chromium Single Cell 3ʹ Feature Barcode Library Kit, 16 rxns PN-1000079; Chromium Single Cell B Chip Kit, 48 rxns PN-1000073; Chromium Single Cell B Chip Kit, 16 rxns PN-1000074; Chromium i7 Multiplex Kit, 96 rxns PN-120262; Product list for generating Chromium Single Cell 3 ʹ Gene Expression Libraries: REAGENT KITS REACTIONS PART NUMBER (PN) Chromium Next GEM Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3. Chromium™ Single Cell V(D)J Enrichment Kit, Human B Cell (store at −20°C) 1000016 Human B Cell Mix 1 6 2000035 Human B Cell Mix 2 6 2000036 Chromium™ Single Cell A Chip Kits PN-120236 & PN-1000009 Product Components # Part Number Chromium™ Single Cell A Chip Kit, 48 rxns (store at ambient temperature) 120236 Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236 Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium™ i7 Multiplex Kit, 96 rxns PN-120262 . 1. 1 User Guide with Feature Barcoding technology for Cell Surface Protein Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Chromium i7 Multiplex Kit, 96 rxns PN-120262 Chromium i7 Multiplex Kit N, Set A, 96 rxn PN-1000084 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. 1000251. FOR USE WITH Chromium Single Cell ATAC Library & Gel Bead Kit, 16 rxns PN-1000 110 Chromium Single Cell ATAC Library & Gel Bead Kit, 4 Chromium Next GEM Chip H Single Cell Kit, 16 rxns PN-100016 2 (store at ambient temperature) Single Index Kit N Set A, 96 rxns PN-1000212 (store at −20°C) Introduction. Click to TOC Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient temperature) Introduction Dual Index Kit TT Set A, 96 rxns PN-1000215 (store at −20°C) Dual Index Kit TT Chromium Next GEM Chip H Single Cell Kit, 48 rxns PN-1000161 Chromium Next GEM Chip H Single Cell Kit, 16 rxns PN-1000162 Chromium i7 Multiplex Kit N, Set A, 96 rxns PN-1000084. Click to TOC Chromium Single Cell 3 Reagent Kits v3 User Guide with Feature Barcoding technology for Cell Surface Protein | Chromium™ Single Cell 3’ Reagent Kits User Guide . 96 rxns PN-1000251. Chromium Next GEM Single Cell 3. Core Scaling comparative lung cancer studies using Chromium Single Cell Gene Expression Flex. 1, 16 rxns PN-1000175 ATAC Buffer B 1 2000193 ATAC Enzyme 1 2000123 20X Nuclei Buffer 1 2000207 Barcoding Reagent B 1 2000194 Barcoding Enzyme 1 2000125 SI-PCR Primer B 1 2000128 Reducing Agent B 1 2000087 Amp Mix 1 2000047 Chromium Next GEM Automated Single Cell Kit, cDNA Generation Module 1, 24 rxns PN-1000395 (store at 4°C) Chromium Next GEM Chip K Automated Single Cell Kit, 48 rxns PN-1000289 (store at ambient temperature) 10xGenomics. Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236 Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium™ i7 Multiplex Kit, 96 rxns PN-120262 . Upgrading to Firmware 4. Click here for the Chromium™ Controller Firmware Update Procedure. Chromium Next GEM Chip H Single Cell Kit, 16 rxns PN-100016 2 (store at ambient temperature) Single Index Kit N Set A, 96 rxns PN-1000212 (store at −20°C) Introduction. Thermal cycler. NOTICES _____ The Chromium Single Cell Chip Kit is a lab equipment product offered by 10x Genomics. NOTICES _____ Chromium™ Single Cell 3’ Reagent Kits v2 User Guide • Rev C Click to TOC i Notices Chromium Single Cell A Chip Kit, 48 rxns PN-120236 Chromium Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium i7 Multiplex Kit, 96 rxns PN-120262. 1, 4 rxns PN-1000128 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127. Chromium GEM-X Single . Click to TOC Chromium Next GEM Single Cell 5' v2 (Dual Index) with Feature Barcode technology Rev A 12 Chromium Next GEM Chip H Single Cell Kit 48 rxns 16 rxns 1000161 1000162 Chromium i7 Multiplex Kit N, Set A 96 rxns 1000084 INSTRUMENT Chromium Controller & Next GEM Accessory Kit - 120223 (12 month warranty) 120246 (24 month warranty) SOFTWARE Cell Ranger ATAC (DOWNLOAD) Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234 Chromium Next GEM Chip J Single Cell Kit, 16 rxns PN-1000230 Single Index Kit N Set A, 96 rxns PN-1000212 Dual Index Kit TT Set A, 96 rxns PN-1000215 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. 2001213 ; Recovery Agent . Chromium Next GEM Chip J Single Cell Kit, 16 rxns PN-1000230. 1 (Dual Index) User Guide Rev B 10 Chromium Single Cell 3’ Reagent Kits v3 Chromium Single Cell 3’ Feature Barcode Library Kit Chromium i7 Multiplex Kit Chromium Next GEM Single Cell 3’ Chromium Next GEM Chip G Single Cell Kit 48 rxns: 16 rxns 1000120: 1000127 Chromium Next GEM Single Cell 3’ Library Construction Kit : v3. 1 User Guide with Feature Barcoding technology for CRISPR Screening • Rev B. Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. Product list for generating Chromium Single Cell 3 ʹ Gene Expression Libraries: REAGENT KITS REACTIONS PART NUMBER (PN) Chromium Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3 16 rxns 4 rxns 1000075 1000092 Chromium Chip B Single Cell Kit Chromium Chip B Single Cell Kit 48 rxns 16 rxns 1000073 1000074 Chromium i7 Multiplex Kit 96 rxns 120262 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127; Single Index Kit T Set A, 96 rxns PN-1000213; Chromium Single Cell 5' Feature Barcode Library Kit, 16 rxns PN-1000080; Single Index Kit N Set A, 96 rxns PN-1000212; Document Type Technical Note. 1 4 rxns PN-1000322 (store at −20°C) Chromium Next GEM Single Cell 3 ʹ LT GEM Kit v3. Click to TOC Chromium Next GEM Single Cell ATAC Reagent Kits v2 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Click to TOC Chromium Single Cell 3 Reagent Kits v3 User Guide with Feature Barcode technology for CRISPR Screening Product list for generating Chromium Single Cell 3 ʹ Gene Expression Libraries: REAGENT KITS REACTIONS PART NUMBER (PN) Chromium Next GEM Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3. The method comprises an effective and accessible strategy for examining genomic alterations and tumor subclonal structures, enabling tumor evolution studies that can inform therapeutic development. 1. Warranty 120212 1 Instrument $75,000 Chromium™Single Cell AChip Kit 120236 48 samples $1,440 Chromium™ Single Cell5’ GelBead & Library Kit 1000006 16 reactions $20,000* Chromium™Single Cell V(D)J Enrichment Kit, Human T Cell 1000005 96reactions $250 Chromium™ i7 Multiplex Kit 120262 96 Single Cell 3’ Reagent Kits v3. Approximately 500 nuclei were targeted using the Chromium Next GEM Chip H Single Cell to generate libraries, and two The basic chemistry that drives the previous iteration of our Chromium Single Cell Gene Expression and Single Cell Immune Profiling assays, powered by Next GEM technology, remains the same in our GEM-X assays—it is related to the foundational Drop-seq method published by Harvard researchers in 2015 (5). Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 1 BC PN-1000474. com Chromium Next GEM Chip K & Gaskets Chromium Next GEM Chip K 6 Chromium Next GEM Automated Single Cell Kit, cDNA Module 2, 24 rxns PN-1000396 (store at -20°C) Chromium Next GEM Chip K Automated Single Cell Kit, 48 rxns PN-1000289 (store Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3. 1 RT Reagent B 1 2000165 RT Enzyme C 1 2000085 Template Switch Oligo 13000228 Reducing Agent B 1 2000087 Cleanup Buffer 2 . Gel Beads (from Chromium Single Cell 3′ Gel Bead Kit v3). Click to TOC Chromium Next GEM Single Cell 5' v2 (Dual Index) with Feature Barcode technology Rev A 12 Chromium Next GEM Single Cell 3ʹ LT Kit v3. Click to TOC Chromium Next GEM Single Cell ATAC Reagent Kits v2 Chromium Single Cell V(D)J Enrichment Kit, Mouse B Cell, 96 rxns PN-1000072 Chromium Single Cell A Chip Kit, 48 rxns PN-1000152 (America & Asia Pacific), PN-120236 (Europe, Middle East & Africa) Chromium Single Cell A Chip Kit, 16 rxns PN-1000151 (America & Asia Pacific), PN-1000009 (Europe, Middle East & Africa) Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient temperature) Introduction Dual Index Kit TT Set A, 96 rxns PN-1000215 (store at −20°C) Dual Index Kit TT Set A Dual Index Plate TT Set A 1 3000431 # PN. Single Cell 3ʹ LT Reagent Kits v3. Chromium Chip B Single Cell Kit, 16 rxns PN-1000074 (store at ambient temperature) Chromium i7 Multiplex Kit, 96 rxns PN-120262 (store at −20°C) Introduction. Capture molecular readouts of cell activity in multiple dimensions, including gene expression, cell surface proteins, immune clonotype, antigen specificity, and chromatin accessibility. 16 or higher is required before running this assay. 1, 16 rxns PN-1000175 ATAC Buffer B 1 2000193 ATAC Enzyme 1 Chromium Single Cell A Chip Kit, 48 rxns PN-120236 Chromium Single Cell A Chip Kit, 16 rxns PN-1000009 Chromium i7 Multiplex Kit, 96 rxns PN-120262 Chromium i7 Multiplex Kit N, Set Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient temperature) Chromium i7 Multiplex Kit, 96 rxns PN-120262 (store at −20°C) Introduction. Dual Index Kit TT Set A, 96 rxns PN-1000215. Dual Index Kit TT Set A, 96 rxns PN-1000215 Chromium Next GEM Single Cell 3 v3. with cells, Chromium X series instruments automate the most critical steps in single cell assays: partitioning and barcoding. Click to TOC. 96 rxns PN-1000215. Chromium GEM-X Single Cell 3' Chip Kit v4, 4 chips PN-1000690 Dual Index Kit TT Set A, 96 rxns PN-1000215 WARNING: Firmware FW 2. Multiplex-compatible Chromium Next GEM Single Cell Fixed RNA Human Transcriptome Probe kits (PN-1000420/1000456) contain 4 or 16 probe sets, Chromium Next GEM Chip Q Single Cell Kit, 48 rxns PN-1000418 / 16 Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3. 1, 16 rxns PN-1000121 Chromium Next GEM Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3. n/a n/a n/a CG000075 Rev A 10xGenomics. associate individual reads back to the individual partitions. Chromium Next GEM Chip H Single Cell Kit, 48 rxns PN-1000161 Chromium Next GEM Chip H Single Cell Kit, 16 rxns PN-1000162 Single Index Kit N, Set A, 96 rxns PN-1000212 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Click to TOC Chromium Next GEM Single Cell 5' v2 (Dual Index) User Guide Rev A 11 Chromium™ Single Cell V(D)J Reagent Kits User Guide . 1: Chromium Next GEM Chip G Automated Single Cell Kit, 48 rxns PN-1000136 Chromium Next GEM Chip G Automated Single Cell Kit, 16 rxns PN-1000146: 50% Glycerol 2000109 ! " # $ # # % & ' " ( # ) $ Click to TOC Chromium Single Cell DNA Training Kit User Guide | Rev B 6 Chromium Single Cell DNA Training Kit, 4 rxns PN-1000061 Introduction Chromium Single Cell DNA Training Kit Chip C Single Cell DNA 2 2000030 Gasket 1 3000072 10xGenomics. 1, 4 rxns PN-1000325 3’ Feature Barcode Kit, 16 rxns PN-1000262 Chromium Next GEM Chip L Single Cell Kit, 16 rxns PN-1000321 3’ CellPlex Kit). FOR USE WITH: Chromium Next GEM Training Reagents, Gel Beads & Chip Kits, 48 rxns PN-1000143 1:1 mixture of human GM12878 and mouse EL4 cells following the Chromium Next GEM Single Cell ATAC Reagent Kits v1. Upgrading to Firmware 4. Chromium Chip D Single Cell DNA Kit, 48 rxns PN-1000042 (store at ambient temperature) Chromium i7 Multiplex Kit, 96 rxns PN-120262 (store at −20°C) Introduction Chromium Chip D Single Cell DNA Kit, PN-1000021 (2 kits are shipped) Click to TOC Chromium Single Cell DNA Reagent Kits User Guide | Rev C 12 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient temperature) Introduction Dual Index Kit TT Set A, 96 rxns PN-1000215 (store at −20°C) Dual Index Kit TT Set A Dual Index Plate TT Set A 1 3000431 # PN. Chromium. 1(Dual Index) User Guide • Rev E. 00 is required before running this assay. Transferring GEMs 18 2. Click to TOC Chromium Next GEM Single Cell 3 v3. 1, 4 rxns PN-1000269 3’ Feature Barcode Kit, 16 rxns PN-1000262 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127. Last Modified February 5, 2020. It is designed to enable single-cell analysis by providing the necessary components to process and profiling 500-10,000 individual cells per sample. Dual Index Kit TS Set A . 4. 8 Chromium Next GEM Single Cell 3’ Kit v3. Partitioning Oil B . 1, 4 rxns PN-1000325 Chromium Next GEM Single Cell 3 ʹ GEM LT Kit v3. 1, 16 rxns PN-1000175 ATAC Buffer B 1 2000193 ATAC Enzyme 1 2000123 20X Nuclei Buffer 1 2000207 Barcoding Reagent B 1 2000194 Barcoding Enzyme 1 2000125 SI-PCR Primer B 1 2000128 Reducing Agent B 1 2000087 Amp Mix 1 2000047 Chromium Next GEM Chip H Single Cell Kit v1. 1, 24 rxns PN-1000141 Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286; Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287; Dual Index Kit TT Set A, 96 rxns PN Chromium Chip E Single Cell ATAC Kit, 16 rxns PN-1000156 (America & Asia Pacific), PN-1000086 (Europe, Middle East & Africa) Chromium i7 Multiplex Kit N, Set A, 96 rxns PN 10xGenomics. The location of the rows labeled 1-3 and J Enrichment Kit, Mouse B Cell Chromium Single Cell V(D)J Enrichment Kit, Mouse T Cell 96 rxns 96 rxns 96 rxns 96 rxns 1000016 1000005 1000072 1000071 Chromium Next GEM GEM generation in Chromium Next GEM Chip G (B). 1(Dual Index) User Guide Rev F 10 Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit TN Set A, 96 rxns PN-1000250 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. ted emulsion-safe pipette tips for all Single Cell protocols. 1 FOR USE WITH Chromium Next GEM Automated Single Cell 3’ Library and Gel Bead Kit v3. 04% BSA (700–1,200 living cells per ml as determined using the LUNA-FL cell counter) and Chromium Next GEM Chip N Single Cell Kit* 80 rxns PN-1000357 | 16 rxns PN-1000375 (*Included with Chromium Next GEM Single Cell 5' HT Kit v2) 5' Feature Barcode Kit, 16 rxns PN-1000541; Dual Index Kit TT Set A, 96 rxns PN After counting, 8 000 cells for each sample (targeted cell recovery) were processed using Chromium Single Cell 3’ GEM, Library & Gel Bead Kit v3 (10x Genomics; PN-1000092), the Chromium Chip B Single Cell Kit (10x Genomics; PN- 1000073), and the Chromium i7 Multiplex Kit v2 (10x Genomics; PN-120262) for samples d2-d15 and Chromium Next GEM Single Cell Chromium Next GEM Single Cell 3’ HT v3. Running the Chromium™ Controller 18 2. ! Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NN Set A, 96 rxns PN-1000243 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. USER GUIDE Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 (store at ambient temperature) 10xGenomics. 1 (Dual Index) User Guide Rev C 10 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Last Modified June 9, 2023. The Chromium Controller is compatible with our suite of Chromium Single Cell products, including Single Cell Gene Expression, Single Cell Immune Profiling, and Single Cell ATAC. 1 (Dual Index) with Feature Barcode technology for Cell Surface Protein and Cell Multiplexing FOR USE WITH Chromium Next GEM Single Cell 3 ’ Kit v3. com Chromium Next GEM Chip K & Gaskets Chromium Next GEM Chip K 6 Chromium Next GEM Chip H Single Cell Kit v1. Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient temperature) Introduction Dual Index Kit TT Set A, 96 rxns PN-1000215 (store at −20°C) Dual Index Kit TT Set A Dual Index Plate TT Set A 1 3000431 # PN. PNs . 1 RT Reagent B 1 2000165 RT Enzyme C 1 2000085 Template Switch Oligo 13000228 Reducing Agent B 1 2000087 Cleanup Buffer 2 Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 Dual Index Kit TT Set A, 96 rxns PN-1000215 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Chromium Next GEM Automated Single Cell 5’ Reagent Kits v2: Chromium Next GEM Chip K Automated Single Cell Kit, 48 rxns PN-1000289 Chromium Next GEM Chip K Automated Single Cell Kit, 16 rxns PN-1000297: 50% Glycerol 2000109 ! " # $ # # % & ' " ( # ) $ Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Click to TOC # PN. Library Construction: End Repair & A-tailing 33 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NN Set A, 96 rxns PN-1000243 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. 1 4 rxns PN-1000130 (store at −20°C) Chromium Next GEM Single Cell 3 ’ GEM Kit v3. The Chromium Next GEM Chip G is assembled in the Chromium Next GEM Secondary Holder. GEM-X Flex Gene Expression Chip Kit, 4 chips. Temperature-controlled heat block. Chromium GEM-X Single Cell 3' Chip Kit v4, 4 chips (PN1000690) Reagents . com Chromium Next GEM Training Chip Kit, 48 rxns PN-1000145 (store at ambient temperature) Chromium Next GEM Training Reagents, Gel Beads & Chip Kit, 48 rxns PN-1000143 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 (store at ambient temperature) Introduction Dual Index Kit TT Set A, 96 rxns PN-1000215 (store at −20°C) Dual Index Kit TT Set A Dual Index Plate TT Set A 1 3000431 # PN. 1 16 rxns PN-1000123 (store at −20°C) Chromium Next GEM Single Cell 3 ʹ GEM Kit v3. com Chip (gasket attached) Chromium Partitioning Oil # PN The Chromium Single Cell ATAC Solution provides a comprehensive, scalable approach to determine the regulatory landscape of chromatin in hundreds to thousands of cells in a single sample. 1, 4 rxns PN-1000128 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 6 Technical Note Chromium Next GEM Single Cell 5' v2: Reagent, Workflow & Performance Updates Rev A Figure 4. Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286 Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287 Dual Index Kit TT Set A, 96 rxns PN-1000215 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. Median V(D)J sequences recovered per cell for TRA/TRB (A) and IGH/IGK/IGL (B) transcripts across samples processed with v1. Cell 5' Kits v3 . 1000215. 2 Post GEM-RT Cleanup and cDNA Amplification. 6 ; 230008 . CG000389 Rev B USER GUIDE Chromium Next GEM Single Cell 3’ Reagent Kits v3. GEM-RT Incubation 20 Protocol Step 3 21 Chromium™ Single Cell 3’ Reagent Kit User Guide Click to TOC iii 4. REACH: Regulation (EC) No 1907/2006 (in particular as Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Chromium i7 Multiplex Kit, 96 rxns PN-120262 Chromium i7 Multiplex Kit N, Set A, 96 rxn PN-1000084. The following protocol describes the steps for single cell mRNA-seq, but the platform is capable of y profiling 500-10,000 individual cells per sample. USER GUIDE. Like most great science, the process begins Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Single Index Kit T Set A, 96 rxns PN-1000213 Single Index Kit N Set A, 96 rxns PN-1000212 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. nomics and are highly recommended for all single cell assays. Dual Index Kit NT Set A, 96 rxns PN-1000242. 220016 : Page 1/6 . 1, 4 rxns PN-1000269 3’ Feature Barcode Kit, 16 rxns Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120; Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127; Dual Index Kit TT Set A, 96 rxns PN-1000215; Dual Index Kit NN Set A, 96 rxns PN-1000243; Dual Index Kit NT Set A, 96 rxns PN-1000242; Document Type User Guide. 3. The Chromium Single Cell B Chip Kit is a laboratory equipment product designed for single-cell analysis. 16 rxns PN-1000419. Available for use only with the Chromium Next GEM Single Cell ATAC Reagent Kits v1. Cell 3' Kits v4 . The following protocol describes the steps for single cell mRNA-seq, but the platform is Chromium Single Cell B Chip Kit, 16 rxns PN-1000074 Chromium i7 Multiplex Kit, 96 rxns PN-120262 Available for use only with the indicated Chromium Controller (PN-120223;120246) or We use the 10X Chromium Controller platform to encapsulate and barcode single cells. 56M cells run on a single chip, for large-scale experiments at less than $0. Post Shearing Size Selection – SPRIselect 32 4. Chromium Single Cell 3 ʹ GEM, Library & Gel Bead Kit v3 16 rxns 4 rxns 1000075 1000092 Chromium Chip B Single Cell Kit Chromium Chip B Single Cell Kit 48 rxns 16 rxns 1000073 1000074 Chromium i7 Multiplex Kit 96 rxns 120262 INSTRUMENT Chromium Single Cell Controller & Accessory Kit 120223 Chromium Controller & Accessory Kit 120263 SOFTWARE Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127 Dual Index Kit TT Set A, 96 rxns PN-1000215 Dual Index Kit NN Set A, 96 rxns PN-1000243 Dual Index Kit NT Set A, 96 rxns PN-1000242 Next GEM reagents are specific to Next GEM products and should not be used interchangeably with non-Next GEM reagents. schulxpokjptpaqncftrrnubsrtvfqgtfphurfzecklnym