Sureselect xt protocol. A streamlined workflow with a 8-hour TAT.

Sureselect xt protocol This guide provides an optimized protocol for preparation of target-enriched Illumina paired-end multiplexed sequencing libraries using SureSelectXT Low Input Reagent Kits. The SureSelect Human All Exon V8 is compatible with the streamlined SureSelectXT HS2, SureSelectXT HS, SureSelectXT Low Input, and SureSelectQXT library preparation and target enrichment systems, which feature a fast, 90-minute hybridization protocol, as well as the legacy SureSelectXT system. Use of a reference This Quick Start Protocol provides key protocol details for experienced users. 3. 10 Safety Notes . SureSelect Library Prep & Target Enrichment Solution Product Name SureSelectXT HS SureSelectXT Low Input SureSelectXT SureSelectXT2 SureSelectQXT DNA Input 10 ng - 200 ng 10 ng - 200 ng 200 ng - 3 µg 100 ng - 1 µg 50 ng Turnaround Time 8 hr 8 hr 1. Before You Begin. It targets the coding regions in the transcriptome for gene expression profiling, RNA fusion detection and novel transcript discovery. Once loaded, the Magnis NGS Prep System performs all SureSelect XT Using SureSelect XT RNA Direct protocol (RNA Direct) workflow, we found transcripts to be upregulated or downregulated to similar degrees with similar confidence levels in both the FF and FFPE samples, demonstrating the utility for meaningful gene expression studies with biobank samples of variable quality. SureSelect XT HS2 DNA Reagent Kit 3 SureSelect XT HS2 Reagent Kit is our state-of-the-art library prep and target enrichment solution, which provides a streamlined and flexible workflow, excellent performance and comprehensive features that can satisfy your NGS library prep needs. DNA Fragmentation and Library Preparation Workflow. (NYSE: A) today introduced a new product designed to address key challenges that laboratories encounter when preparing DNA sequencing libraries for their research. Target Enrichment System for Illumina Multiplexed Sequencing 9. See Table 3 on page 14 for ordering information. 65 MB; PDF with a ClearSeq Panel, a SureSelect Exome or a Custom Panel. † Minor update to workflow overview figure on page 10 . See Table 30 50 for the on page hybridization thermal cycler program This document provides users with a complete set of protocols that encompass all Covaris Adaptive Focused Acoustics™ (AFA) instruments for DNA shearing to 150 to 200 bp with 50 to 55 µl of sample volume, to be readily compatible with the Agilent SureSelect Target Enrichment System (XT or XT2). 7 Reference . See 2Table on page 13 for ordering information. † Design ID information added to Table 3 on page 14 for pre-designed SureSelect probes. 1 Before You Begin This chapter contains information that you should read and This guide describes Agilent's recommended operational procedures to capture the genomic regions of interest using Agilent's SureSelectXT Target Enrichment Kit for Illumina Multiplex Sequencing. Target Enrichment System for Illumina Multiplexed Sequencing 7. For Research Use Only. † Design ID information added to Table 2 on page 13 for The new SureSelect Enzymatic Fragmentation Kit is an endonuclease-based enzymatic shearing method which generates random DNA fragments from samples of varying quality and input amounts using a single protocol. Pay close SureSelect XT HS2 RNA System using Pre-Capture Pooling Protocol 5 What’s New in Version C0 † Support for SureSelect XT HS Human All Exon V8+UTR Probe. The workflow is compatible with automation and unique dual sample indexes. It calls attention to an operating procedure, practice, or the like that, if not correctly per- The new SureSelect Enzymatic Fragmentation Kit is an endonuclease-based enzymatic shearing method which generates random DNA fragments from samples of varying quality and input amounts using a single protocol. The V6 sample underwent the SureSelect XT target enrichment protocol with an overnight hybridization, while the V7 sample was processed with the SureSelect XT HS target enrichment protocol (which uses a 90 minute fast hybridization). The SureSelect XT HS2 mRNA Library Preparation Kit is Agilent’s newest strand-specific, poly-A enriched mRNA-Seq solution for Illumina sequencing. Once loaded, the Magnis NGS Prep System performs all SureSelect XT Protocol Considerations for the SureSelect Cancer All-In-One Assay Perform library preparation and target enrichment using a SureSelect Cancer All-In-One panel according to the instructions in the SureSelectXT HS system protocol or the SureSelectXT Low Input system protocol, using the additional considerations listed below. Protocol for Magnis NGS Prep System-automated preparation of SureSelect XT HS target-enriched NGS libraries for Illumina paired-end multiplexed sequencing. Visit SureSelect XT HS2 DNA System Protocol for the full User Guide with further information including: – Safety information – Notices to purchaser – List of required materials – FFPE sample qualification guidelines – QC output examples 8 SureSelect XT HS2 mRNA Library Preparation using Agilent NGS Workstation Prepare the reagent plates 46 Prepare the water reservoir 47 Load the Agilent NGS Workstation 47 Run VWorks protocol Poly-ASelection_XT_HS2_mRNA 49 Step 2. SureSelectXT Target Enrichment System for Illumina Paired-End Sequencing Library Illumina HiSeq and MiSeq Multiplexed Sequencing Platforms Protocol Version 1. 91 Kit Contents 92 Nucleotide Sequences of † Support for SureSelect XT HS Human All Exon V8+UTR Probe. Enrichment of ThruPLEX libraries with Agilent SureSelect platforms is easily performed. This protocol can be employed either manually NOTE This protocol describes automated sample processing using the Agilent NGS Workstation. A streamlined workflow with a 8-hour TAT. For non-automated sample processing procedures for Agilent's SureSelectXT Target Enrichment Kit for Illumina Multiplex Sequencing, see publication G7530-90000. Not for use in diagnostic procedures. Fragment DNA: Enzymatic or Covaris. This publication provides optimized protocols for workflows that include SureSelect XT HS2 DNA library preparation (+/- duplex molecular barcodes or MBCs) with We adapted the SureSelect XT HS2 DNA protocol to prepare libraries using as input material samples after single-cell Whole Genome Amplification (scWGA), instead of genomic DNA s SureSelectQXT Target Enrichment for Illumina Multiplexed Sequencing 5 What’s New in Version F2 † Support for SureSelect XT HS Human All Exon V8 Probe and updated recommendation for use of SSel XT HS and XT Low Input Human All Exon V7 Probe (see Table 2 on page 14) † Updates to instructions in the “Hybridization and SureSelect XT HS2 DNA System Protocol using NGS Bravo Option A 3 Safety Notices CAUTION A CAUTION notice denotes a hazard. User Manuals; English; 15 Nov 2021; 4. The Magnis SureSelect XT HS2 DNA Reagent kit, combined with the Magnis NGS Prep system, provides an easy, fully automated walkaway workflow for target enrichment and NGS library preparation. The chart below details the reagents necessary for this SureSelect XT protocol. 6 Appendix: Using FFPE-derived DNA Samples . 12 Materials Required . This Quick Start Protocol provides key protocol details for experienced users. SureSelect XT HS2 DNA System Protocol Agilent Technologies 1. Not for use in Diagnostic Procedures. It enables the enzymatic fragmentation workflow for the SureSelect XT HS and SureSelect XT Low Input Reagent Kits. Downstream Sequencing Modifications 87 Modifications for samples assessed using Agilent’s Genomic DNA ScreenTape 89 SureSelect. See Table 2 on page 13 for ordering information and see Table 28 on page 49 for the hybridization thermal cycling program recommended for the SureSelect XT HS Human All Exon V8 probes with the SureSelect XT HS2 RNA system. Visit SureSelect XT HS2 mRNA System Protocol for the full User Guide with further information including: – Safety information – Notices to purchaser – List of required materials – Sample qualification guidelines – QC output examples SureSelectXT Low Input Automated Target Enrichment for the Illumina Platform Automated using Agilent NGS Workstation Option B Protocol Version G3, December 2022 SureSelect platform manufactured with Agilent SurePrint technology. The the SureSelect All Evon V6 or SureSelect All Exon V7. This chapter contains information (such as procedural notes, safety information, required reagents and equipment) that you should read and understand before you start an experiment. Human Methyl-Seq Capture Library * 16 reactions 96 reactions 480 reactions * Use of other SureSelect Capture Libraries is not supported by this protocol and requires optimiza-tion of hybridization and post We adapted the SureSelect XT HS2 DNA protocol to prepare libraries using as input material samples after single-cell Whole Genome Amplification (scWGA), instead of genomic DNA samples, for library preparation for Illumina single-cell Whole Genome Sequencing (scWGS). 39 MB; PDF SureSelect XT HS2 DNA Protocol using Magnis NGS Prep System 8 Overview of the Workflow The workflow for SureSelect XT HS2 DNA target enrichment using the Magnis NGS Prep System is summarized in Figure 1. See Table 2 on page 13 for ordering information. RNA samples, pre-plated reagents and labware are loaded on the instrument. This protocol is specifically developed and optimized to enrich targeted regions of the transcriptome and minimize Protocol for SureSelect XT HS2 DNA Library Preparation and Overnight-Hyb, post-capture pooling Target Enrichment for Illumina paired-end multiplexed sequencing. 10 Required Reagents. SureSelect XT Methyl-Seq Target Enrichment System for Illumina Multiplexed Sequencing Protocol. Table 1 Compatible SureSelect Platforms SureSelect platform Workflow options Protocol document number SureSelect XT HS2 DNA Post-capture pooling G9983-90000 Pre-capture pooling G9985-90000 SureSelect XT HS2 DNA Kits and Supported Workflows SureSelect XT HS2 DNA kits are available in a variety of kit configurations supporting several workflow options. Fragment the mRNA samples 50 Prepare the workstation for protocol Fragmentation_XT_HS2_mRNA 50 SureSelect XT HS2 DNA Kits and Supported Workflows SureSelect XT HS2 DNA kits are available in a variety of kit configurations supporting several workflow options. Protocol Modifications 94 Downstream Sequencing Modifications 94 Modifications for samples assessed using the Agilent NGS FFPE QC Kit 95 SureSelect. Pay close attention USER’S GUIDE: AGILENT SURESELECT XT TARGET ENRICHMENT ON THE SCICLONE NGS WORKSTATION Agilent ® SureSelectXT Target Enrichment System for Illumina Paired-End Sequencing Libraries with Multiplexing (Protocol v1. XT. This publication provides optimized protocols for workflows that include SureSelect XT HS2 DNA library preparation (+/- duplex molecular barcodes or MBCs) with • New table summarizing SureSelect XT HS2 DNA Reagent Kit format options (Table 2 on page 10) • Support for SureSelect XT HS PreCap Clinical Research Exome V4 (Table 3 on page 11 and Table 31 on page 35) • Updates to probes supported using this workflow (see Table 3 on page 11). Quick Start Protocol. Oct 31, 2023 · We adapted the SureSelect XT HS2 DNA protocol to prepare libraries using as input material samples after single-cell Whole Genome Amplification (scWGA), instead of genomic DNA s 4 SureSelect XT HS2 DNA System Protocol using Agilent NGS Workstation What’s New in Version B2 • Design ID information added to Table 4 on page 15 for pre-designed SureSelect probes. See Table 28 48 for on pagepre-capture pooling recommendations. Protocol Modifications 89 Downstream Sequencing Modifications 90. Capture understanding of SVs. Sa mple processing steps SureSelect XT HS2 RNA Protocol using Magnis NGS Prep System 8 Overview of the Workflow The workflow for SureSelect XT HS2 RNA target enrichment using the Magnis NGS Prep System is summarized in Figure 1. 5 day 7 hours Covaris SureSelect XT HS Target Enrichment using the Magnis NGS Prep System Protocol. Methyl-Seq Reagent Kit 16 reactions 96 reactions 480 reactions Agilent p/n G9651A p/n G9651B p/n G9651C SureSelect. SureSelect XT Automated Library Prep and Capture System for the Illumina Platform Protocol. Both samples showed comparable performance Pre-Capture Pooling for SureSelectXT HS / SureSelectXT Low Input Target Enrichment System 5 What’s New in Version E0 † Support for SureSelect XT HS Human All Exon V8+UTR and SureSelect XT HS Human All Exon V8+NCV Probes. Visit SureSelect XT HS2 RNA System Protocol for the full User Guide with further information including: – Safety information – Notices to purchaser – List of required materials – FFPE sample qualification guidelines – QC output examples SureSelect XT Methyl-Seq Target Enrichment System for Illumina Multiplexed Sequencing Protocol. User Manuals; English; 25 Apr 2018; 1. In this application note, SureSelectXT is applied to nanopore long-read sequencing, enriching for CDKN2A and SMAD4 tumor suppressor genes, to improve the depth and variant calling accuracy of nanopore sequencing. XT . This application note focuses on optimizing the SureSelectXT protocol to long-read sequencing SureSelect. 19 This protocol differs from the Illumina Multiplexed Paired-End sequencing manual and other SureSelect protocols at several steps. The modules marked in red are not required when integrating with ThruPLEX kits. 93 Modifications for all FFPE DNA samples 94 SureSelect. Pay close SureSelectXT2 Target Enrichment System for the Illumina Platform Featuring Pre-Capture Indexing Reagents and Protocols Protocol Version H0, August 2020 SureSelect platform manufactured with Agilent SurePrint Technology For Research Use Only. Hybridize libraries to probe. For SureSelect XT SureSelect XT Low Input Reagent Kits enable libraries from 10 ng DNA input from intact or FFPE samples. 11 Required Equipment . SureSelect XT HS2 DNA with Pre-capture Pooling. Protocol for SureSelect XT Automated Library Prep and Capture System for Illumina on the NGS Workstation. Hybridization/Capture Workflow. SureSelect XT HS2 DNA Kits Protocol 9 1 Before You Begin Procedural Notes 10 Safety Notes 10 SureSelect XT HS2 DNA Kits and Supported Workflows 11 Additional Materials Required 13 Make sure you read and understand the information in this chapter and have the necessary equipment and reagents listed before you start an experiment. NOTE This protocol describes automated sample processing using the Agilent NGS Workstation. Procedural Notes. This guide provides an optimized protocol for Illumina paired-end multiplexed library preparation using the SureSelectXT Library Prep and Capture System. DNA samples, pre-plated reagents and labware are loaded on the instrument. G9985-90500 Rev A0 **For Research Use Only. See “Appendix 2: Use of Run-Time Prepared Probe Strips Jan 27, 2020 · Enhanced performance and flexibility to power cancer and genetics research Agilent Technologies Inc. User Manuals; English; 03 Dec 2021; 2. SureSelect XT HS2 DNA Protocol using NGS Workstation Option B+ with ODTC 3 In this Guide This guide provides an optimized protocol for preparation of target-enriched Illumina paired-end multiplexed sequencing libraries using SureSelect XT HS2 Reagent Kits. SureSelect XT HS outperforms other library prep solutions at 100X coverage, especially as sample quality decreases: % bases covered by at least 100X read depth is plotted against gDNA of decreasing quality (qPCR-based gDNA quality (ddCq) and is calculated using Agilent’s FFPE QC Assay kit SureSelectXT RNA Direct Protocol Agilent Technologies 1. The SureSelectXT Low Input Reagent Kits and protocol support molecular barcoding of indexed library samples prior to target enrichment and amplification. 12 Make sure you read and understand the information in this chapter and have the necessary equipment and reagents listed before you start an experiment. Flexible sample requirements allow you to start from either pre-fragmented DNA, or enzymatically fragment your gDNA samples on-deck. 13 Optional Materials. SureSelectXT Low Input Target Enrichment System with Dual Indexing 5 What’s New in Version E0 † Support for SureSelect XT HS Human All Exon V8+UTR and SureSelect XT HS Human All Exon V8+NCV Probes. 4 SureSelect XT HS2 DNA Library Preparation and Target Enrichment In this Guide This guide provides an optimized protocol for preparation of target-enriched Illumina paired-end multiplexed sequencing libraries using the SureSelect XT HS2 DNA system. 10 Procedural Notes . 12 Safety Notes. SureSelect XT HS and XT Low Input Enzymatic Fragmentation Kit 3 In This Guide… This guide provides an optimized protocol for enzymatic fragmentation of gDNA samples, replacing mechanical shearing of gDNA samples, prior to DNA library preparation for next-generation sequencing (NGS) using either SureSelect XT HS Reagent Kits or SureSelect XT Low This guide provides an optimized protocol for preparation of target-enriched Illumina paired-end multiplexed sequencing libraries using SureSelectXT Low Input Reagent Kits. 5 day 1. Key Features •384 unique dual sample indexing (UDI) SureSelect XT HS outperforms other library prep solutions at 100X coverage, Hybridize using SureSelectXT HS reagents and protocol Capture hybrids on magnetic beads SureSelect. The new Agilent SureSelect XT HS2 DNA Kit represents the state-of-the-art in library preparation and target enrichment, offering 根据官方 protocol 进行末端修 复、加 A、加接头和样品纯化步 骤。文库制备缓冲液在使用前需 确保完全解冻（特别是在经过干 冰运输后）和充分混合。 起始量为 25 ng cfDNA 时，Pre-PCR cycle 数目为 8 即可产生足以 进行杂交的产物（SureSelectXT HS 建议的 500–1000 ng 制备文 SureSelect. This chapter contains information that you should read and understand before you start an experiment. • Support for use of Agilent’s Alissa Reporter software for SureSelect XT HS2 DNA library Magnis SureSelect XT HS Rev B. Pour plus d’informations sur la façon de sélectionner le protocole compatible avec le format de votre kit de réactifs et avec les autres paramètres de processus pendant la configuration du cycle, reportez-vous à la page27. 12 MB; PDF SureSelectXT HS Protocol using Magnis NGS Prep System 4 What’s New in Version D0 • Support for Magnis protocols SSEL XTHS-EPIS-RevB-ILM and LT-SSEL XTHS-EPIS-RevB-ILM, set up using a Magnis SureSelect XT HS Rev B Reagent Kit (PN G9730D), supplied with empty probe input strips (EPIS). Overview of the Workflow. Protocol Considerations for the SureSelect Cancer All-In-One Assay Perform library preparation and target enrichment using a SureSelect Cancer All-In-One panel according to the instructions in the SureSelectXT HS system protocol or the SureSelectXT Low Input system protocol, using the additional considerations listed below. Prepare streptavidin beads. 4 SureSelect XT RNA Target Enrichment for Illumina In this Guide This guide describes an optimized protocol for Illumina paired-end multiplexed library preparation using the SureSelectXT RNA Target Enrichment system. 2 May 2011) NOTE The SureSelect Enzymatic Fragmentation Kit has not been optimized for use with the SureSelectXT, SureSelectQXT or SureSelectXT2 systems. 1, February 2012 SureSelect platform manufactured with Agilent SurePrint Technology Research Use Only. NOTE This protocol differs from other SureSelect protocols at several steps. This boosting algorithm is specifically optimized for the hybridization conditions in the SureSelect XT HS2 library preparation protocol to deliver optimal sequencing uniformity and read depth across the full range of GC content percentages. nffaylbr ocn arwhd rvur tqwr ucmgl bjb dpbwpo fseru ozqzuss